登录     注册     忘记密码

详细信息

改良型富血小板纤维蛋白复合双相磷酸钙可促进兔骨髓间充质干细胞的活性  |浏览量:200

Biphasic calcium phosphate loaded with advanced platelet rich fibrin can promote the activity of rabbit bone marrow mesenchymal stem cells

文献类型:期刊文献

中文题名:改良型富血小板纤维蛋白复合双相磷酸钙可促进兔骨髓间充质干细胞的活性

英文题名:Biphasic calcium phosphate loaded with advanced platelet rich fibrin can promote the activity of rabbit bone marrow mesenchymal stem cells

作者:王玉姣[1] 刘丹[2] 孙嵩[3] 孙勇[1]

第一作者:王玉姣

第一机构:西南医科大学口腔医学院|西南医科大学口腔医学院

机构:[1]西南医科大学口腔医学院,四川省泸州市646000;[2]龙泉驿社区卫生服务中心,四川省成都市610015;[3]成都市第一人民医院,四川省成都市610000

年份:2021

卷号:25

期号:4

起止页码:504-509

中文期刊名:中国组织工程研究

外文期刊名:Chinese Journal of Tissue Engineering Research

收录:CSTPCD;;北大核心:【北大核心2017】;

语种:中文

中文关键词:骨;材料;双相磷酸钙;细胞增殖;干细胞;矿化;骨再生

外文关键词:bone;material;biphasic calcium phosphate;cell proliferation;stem cell;mineralization;bone regeneration

分类号:R459.9;R-332

摘要:背景:将生长因子加入支架材料能促进体外培养干细胞的增殖及成骨分化,促进骨组织再生。改良型富血小板纤维蛋白中含有多种生长因子,能够促进多种细胞的增殖活性及相关功能蛋白的表达。目的:观察改良型富血小板纤维蛋白、双相磷酸钙单独或联合使用对骨髓间充质干细胞生长状况的影响。方法:将第3代兔骨髓间充质干细胞分4组培养,分别加入基础培养基(空白组)、改良型富血小板纤维蛋白条件培养基(A-PRF组)、双相磷酸钙(BCP组)、改良型富血小板纤维蛋白条件培养基与双相磷酸钙(A-PRF+BCP组)。CCK-8法检测细胞增殖活性,甲基罗丹明-鬼笔环肽荧光染色观察细胞黏附情况,定量检测细胞内碱性磷酸酶活性及矿化结节形成情况。结果与结论:①A-PRF+BCP组培养3,5,7,11,14 d的细胞增殖吸光度值高于其他3组,A-PRF组培养1,3,5 d的细胞增殖吸光度值高于BCP组、空白组,BCP组培养7,11,14d的细胞增殖吸光度值高于A-PRF组、空白组;②甲基罗丹明-鬼笔环肽荧光染色显示,BCP组、A-PRF+BCP组骨髓间充质干细胞黏附在双相磷酸钙材料表面,并且A-PRF+BCP组细胞与微丝数量明显多于BCP组;③培养1,21,28 d的矿化结节合成情况为:A-PRF+BCP组>BCP组>A-PRF组>空白组;④培养5,7,9 d的细胞碱性磷酸酶含量为:A-PRF+BCP组>BCP组>A-PRF组>空白组,组间两两比较差异有显著性意义(P<0.05);⑤结果表明,双相磷酸钙前期促细胞增殖进作用较弱,但其支架作用良好,改良型富血小板纤维蛋白于前期促进细胞增殖作用明显,二者联合使用时对细胞的增殖分化均有良好促进作用。
BACKGROUND:Adding growth factor to scaffold material can promote the proliferation and osteogenic differentiation of cultured stem cells in vitro and promote bone tissue regeneration.Advanced platelet rich fibrin(A-PRF)contains a variety of growth factors,which can promote the proliferation activity of a variety of cells and the expression of related functional proteins.OBJECTIVE:To observe the effects of the sole and combined usage regarding A-PRF and biphasic calcium phosphate(BCP)on the growth of bone marrow mesenchymal stem cells.METHODS:The third generation of rabbit bone marrow mesenchymal stem cells was cultured in four groups,including basic medium(blank group),A-PRF condition medium(A-PRF group),BCP(BCP group),A-PRF condition medium and BCP(A-PRF+BCP group).Cell proliferation activity was detected by CCK-8 assay.Cell adhesion was observed based on methylrhodamine-phalloidin fluorescence staining.Intracellular alkaline phosphatase expression and mineralized nodules were quantitatively measured.RESULTS AND CONCLUSION:(1)The absorbance value of proliferation of the A-PRF+BCP group was higher than that of the other three groups at 3,5,7,11 and 14 days after culture.The absorbance value of proliferation of cells cultured in the A-PRF group was higher than that of the BCP group and the blank group at 1,3 and 5 days after culture.The absorbance value of proliferation of BCP group was higher than that of the A-PRF group and the blank group at 7,11 and 14 days after culture.(2)Methylrhodamine-phalloidin fluorescence staining showed that bone marrow mesenchymal stem cells in the BCP group and the A-PRF+BCP group adhered to the surface of BCP and the number of cells and microfilaments in the A-PRF+BCP group was higher than that in BCP group.(3)The synthesis of mineralized nodules was A-PRF+BCP group>BCP group>A-PRF group>blank group at 1,21 and 28 days after surgery.(4)The expression of alkaline phosphatase was A-PRF+BCP group>BCP group>A-PRF group>blank group at 5,7 and 9 days.There was significant difference between the two groups(P<0.05).(5)The results showed that BCP exerted a weak influence on promoting cell proliferation in the early stage,but its effect of scaffold was apparent.A-PRF had a significant effect on promoting cell proliferation with considerable influence on promoting cell proliferation and differentiation when combined BCP.

参考文献:

正在载入数据...

版权所有©西南医科大学 重庆维普资讯有限公司 渝B2-20050021-8 
渝公网安备 50019002500408号 违法和不良信息举报中心